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An experienced pathologist marked representative areas of tumour samples on haematoxylin- and eosin- stained tumour slides.
Samples were reviewed by independent neuropathologists who marked representative tumour areas on the back of the slides to be used for evaluation of immunostaining.
For each of these blocks a single hematoxylin and eosin (H&E) slide was prepared on which the same pathologist marked representative areas of malignant and benign tissue.
Tumor samples were reviewed histologically using hematoxylin and eosin staining, and then we marked representative areas more centrally on the paraffin blocks away from hemorrhagic and necrotic areas.
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DAPI nuclear staining identified all cells in the images (Figure 4, top panels) wherein the filled white arrows mark representative dengue-infected cells, and the open white arrows mark representative uninfected cells in all panels.
For the tissue microarray (TMA), corresponding H&E-stained sections from each FFPE block were used to define and to mark representative tumor areas by the pathologist, two to four random, representative 2-mm tissue cores were punched from the designated area using a biopsy needle and inserted in a grid pattern into a recipient paraffin block using a tissue arrayer [ 34].
Our senior pathologist reviewed the slides and marked the representative areas of tumor tissues.
Transcripts were independently read and re-read by two authors (EWS and SEL) and marked for representative themes that reflected the data [ 14].
Each patient's haematoxylin and eosin (H&E) slides were reviewed by an expert pathologist (R. Barbazza), who marked the representative tumor areas to be analyzed.
Each patient's haematoxylin & eosin (H&E) slides were reviewed by an expert pathologist (RB), who marked the representative tumour areas to be analysed.
It also was a disappointment for some of the park's neighbors, who preferred the grass and trees that the turf field replaced, said Marnie Foster-Marks, representative to the Village Board for the Flint Park Neighborhood Association.
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