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Marked orientation dependence of RAG off-target activity within loops spanning up to 2 megabases implies involvement of linear tracking.
Unexpectedly, given the results of previous studies of expansion and the marked orientation dependence of deletion frequencies (Kang et al. 1995; Freudenreich et al. 1997; Miret et al. 1998; Schweitzer and Livingston 1999; Zahra et al. 2007), the frequencies of expansion events were remarkably similar in the two orientations of the repeat array.
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Eyes were marked for orientation, then enucleated and immersion fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.3, at 4°C overnight.
Eyes were marked for orientation, then enucleated, punctured behind the cornea for better penetration of the fixative, and immersion fixed in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB, pH 7.4) for 2 3 hrs (opsin immunocytochemistry) or several days (Nissl staining of ganglion cells).
The superior portion of the cornea was marked for orientation prior to enucleation.
Immediately after death, the superior side of each eye was marked for orientation, and both eyes were enucleated.
The 'scratch' was introduced by scraping the monolayer with a p200 pipette tip and marked for orientation and photographed by a fluorescent microscope immediately and after 24-, 48- and 72 h.
b Probe index mark orientation towards the patient's anterior/front.
Per this standard, a blank drawing sheet should contain the title block, frame for limiting the drawing space, centering marks, orientation marks, metric reference graduation, grid reference system, and trimming marks.
Parameters to define plough mark orientation were defined and measured in the field on boulders with plough marks within a study terrace located in an archaeological area with an approx of 2040 year age.
The yellow arrow marks the orientation of the profile given below.
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CEO of Professional Science Editing for Scientists @ prosciediting.com