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Eyes were marked for orientation, then enucleated and immersion fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.3, at 4°C overnight.
Eyes were marked for orientation, then enucleated, punctured behind the cornea for better penetration of the fixative, and immersion fixed in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB, pH 7.4) for 2 3 hrs (opsin immunocytochemistry) or several days (Nissl staining of ganglion cells).
The superior portion of the cornea was marked for orientation prior to enucleation.
Immediately after death, the superior side of each eye was marked for orientation, and both eyes were enucleated.
The LEEP specimens were marked length-wise with ink and were radially sectioned after the endocervical portion had been marked for orientation.
The 'scratch' was introduced by scraping the monolayer with a p200 pipette tip and marked for orientation and photographed by a fluorescent microscope immediately and after 24-, 48- and 72 h.
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Orientation for single parents, followed by coffee and conversation.
The removed tissue was marked for its orientation and sent for histology.
The excised tumor was carefully marked for location and orientation of spectroscopic PA imaging planes.
After the wire-marked milk duct was dissected, it was suture marked retroareolarly for orientation (Fig. 6).
A method for locating the same nanostructure in different microscopes, using the nanoindentation marks for orientation on the sample, is described.
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