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Areas of interest were identified microscopically and marked for macrodissection.
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All were marked for United Russia.
She has been marked for entry.
This observation is particularly marked for CCR10.
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Regions containing a minimum of 60% tumor cellularity were required for macrodissection, and in most instances tumor cellularity of at least 70 90% was identified for inclusion in the sample for RNA isolation.
These H&Es were used as references for macrodissection of unstained slides.
From each case an H&E stained section that had been previously annotated for cancerous and normal areas by an expert pathologist (DMB) was used as a guide for macrodissection.
In all cases, a 4 micrometers-thick section was stained with hematoxylin & eosin and reviewed by a pathologist before extraction, to confirm the presence of melanoma and to select areas with the highest density of tumor cells for macrodissection.
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