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In our lineage tracing studies presented here, two population of marked cells were evident in the mice 180 days post-TAM: dentate gyrus granule neurons (NeuN+, presumably integrated into hippocampal circuitry), and SGZ Type-1 cells (GFAP+ with radial glial morphology).
The marked cells were seeded onto the HUVEC monolayers and co-cultured for 1 h.
In an experiment in which mice were fed with milk containing marked maternal cells, the marked cells were demonstrated to invade the tissues of offspring [ 18].
Marked cells were analyzed using the flow cytometer GUAVA EasyCyte software to evaluate DNA content of cells and, thus, cell cycle distribution of samples.
We generated groups of flies in which the clonally marked cells were either wildtype (control group) or overexpressing a constitutively activated allele of Egfr (Egfr λtop ) (experimental group) (Queenan et al., 1997).
In control StdMO-injected embryos, marked cells were found in the dorsal axial hypoblast along the entire AP axis at 10.5 hpf (Fig. 8B, upper panels) which is consistent with a previous report [34].
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By contrast, the anterior movement of marked cells was perturbed, exhibiting a shortened axial mesoendoderm in cfl1 tMO1-injected embryos (Fig. 8B, lower panels).
After incubation, erythrocyte lysis and fixation of marked cells was performed using the Immuno-Prep EPICS Kit (Coulter Electronics) and Q-prep Work Station (Coulter Electronics, Miami Lakes, FL).CCR10 expression was evaluated by indirect immunofluorescence staining on PBMC: by incubating 250,000 cells with a goat anti human CCR10 (diluted 1∶25) (Capralogics Inc., Hardwick MA).
Such marked cells are then recognised and eliminated by macrophages in a process involving C1q binding to cell surface expressed calreticulin (Vandivier et al, 2002).
When a population of epithelial cells containing 10% lacZ-positive marked cells was transplanted at limiting dilution, all transplant outgrowths were found to contain mixtures of lacZ-positive and lacZ-negative cells [ 22].
The loss of precision is mostly caused by the fact that, by direct segmentation, a significantly smaller number of marked cells is recognized than by the TV/segmentation method (cf. Figure 2D F) while the ratio of artifacts produced by both methods is comparable.
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