Exact(2)
To determine the fate of the marked cells over longer periods, we examined brains 180 days post-TAM.
In the present case, since mitochondrial DNA mutations led to a steady accumulation of marked cells over time, the effective size distribution of surviving clones involves the sum over all of these histories, and is predicted to take the form (Klein et al., 2010), (1) P n surv.
Similar(58)
Related to Figure 3. DOI: http://dx.doi.org/10.7554/eLife.06303.007 In a further test, we made small clones of marked cells that over-express ds and studied them in the larva in vivo.
Over the next few days, the marked cells produced offspring with similar kinetics to what is seen in Lgr5-based tracing experiments and the rapid appearance of labelled offspring suggests that these are non-quiescent.
Strong expression of the mCitrine reporter, a low density of marked cells, and labeling spanning all cerebellar cortical regions allowed manual segmentation of the dendritic morphology of neighboring granule cells over the entire expanse of the cerebellum.
Multiple reports have cautioned against over-interpretation of results of labeled transplanted cells to host cells, though there has been a dearth of reports quantifying the actual number of activated tissue macrophages engulfing the markers or marked cells [24] [29].
In the SGZ, cells from the Ascl1 lineage also generate neurons over extended periods (30 days) but as the animal ages and neurogenesis decreases, so does the generation of new neurons from Ascl1 marked cells.
Another important issue is viability of marked cells.
Large marked cells in the TC domain of the DT.
Under exposure to fludioxonil the wild-type strain showed marked cell swelling, indicative of intracellular glycerol over-accumulation (Fig. 4B and 4C).
The marked cell types show a significant correlation with prognosis.
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