Expression profiling of fluorescent-activated cell sorting (FACS) sorted CD44HIGH CD24LOW marked cells, a population enriched for breast epithelial stem cells, showed transcripts associated with cell motility, cell adhesion, cell proliferation, chemotaxis and angiogenesis.
In the lineage tracing Isl1-cre R26R Isl1-cre R26Rit has been demouserated that Isl1 model cell populatitns thas contribeen to subsets of ECs in myocardemonstratedthatendothelIsl1
Since γ-RVs and LVs integrate in the cellular genome in a semi-random fashion [ 5- 8], in a population of vector marked cells each clone and its progeny harbor an integrated vector in a unique genomic position that can be used as a distinctive genetic identifier.
Therefore, PCNA can be used to mark cell proliferation and to identify a population of progenitor cells [ 20].
Dotted white lines mark cell nuclei.
Although all of these lines mark basal cell populations, subtle (or not so subtle) differences in their expression levels or activity pattern are to be expected.
Results of this analysis are consistent with ALDH expression marking a stem cell population.
However, the presence of Ascl1 in a subpopulation of SVZ B cells suggests that Ascl1 may be marking a stem cell population, or at least a population with long term neurogenic potential in this region of the adult brain.
Moving back in developmental time, we examined expression of Brachyury (T), a marker for the entire mesodermal lineage which in combination with Flk1 marks the hemangioblast cell population [43].
This induces the expression of the master regulator of pancreatic development, Pdx1 (pancreatic and duodenal homeobox 1), and marks the precursor cell population for pancreatic development.
