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The control group without EGCG treatment produced marked cell migration in the wound area 24 hr after wounding, but wounds treated with EGCG showed significant delays under the same conditions.
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Dotted white lines mark cell nuclei.
Concordant with these data, HT115 cells treated with DZNep also exhibited marked reduction in cell migration as measured using transwell migration assay.
While activated γδ T cells did not migrate towards serum-free medium (Fig. 1B), FBS induced marked γδ T cell migration (~ 15 20% of input cells — data not shown).
Small interfering RNA-mediated downregulation of FXR and guggulsterone-mediated FXR inhibition resulted in a marked reduction in cell migration and invasion.
In addition to promoting motile and invasive activities of Y-79 cells in vitro, the expressions of N-cadherin and vimentin greatly sensitized Y-79 cells to TGF-β1, stimulating marked increases in cell migration and invasion as well as in MMP-2/-9 production.
DOI: http://dx.doi.org/10.7554/eLife.02907.006 EMT is characterized by a number of functional and molecular changes, including marked increase in cell migration and invasion, actin stress fiber formation, upregulation of mesenchymal markers and downregulation of epithelial markers (Thiery et al., 2009).
Individual siRNAs against BCAR3 decreased cell migration, marked by only about a 42% increase in relative wound density after 48 hours.
Red (mCherry) marks cells in S/G2/M phase of the cycle, green (EGFP) marks cilia, blue (DAPI) marks nuclei.
After the process proceeded for roughly five minutes, extensive cell migration commenced, marking the third phase.
In addition, the change in CD4+ cells marked for migration (CX3CR1) was significantly greater than the change in cell volume (P < 0.05; see Figure 2(a)).
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CEO of Professional Science Editing for Scientists @ prosciediting.com