Sentence examples for marked cassette from inspiring English sources

The 9X-Myc epitope tag was amplified from a TRP1 marked cassette (Knop et al. 1999) using DDO1462/-1463 and transformed into wild-type and tdna mutants.

The first one is the question of the additional insertion of the new "marked" cassette during the transduction process, not into the point corresponding to its location in the donor genome, but into the "unmarked" cassette in the recipient chromosome.

Marker cassette (cleaved off from pLRMG-orc1-1 with PstI and SalI) was inserted to the pUC-whip∗ to yield whip∗ replacement plasmid pUC-M-whip∗.

This method "Marker cassette Integration and target gene Deletion (MID)" employs three homologous sequence arms, which are target gene arm (G-arm), left sequence arm (L-arm) and right sequence arm (R-arm).

Because RRN5 gene is an essential gene, a helper plasmid pRRN5 harboring wild type RRN5 gene expression cassette marked with URA3 gene was introduced into SH6471 strain prior to RRN5 gene disruption.

The subsequent recombination event deletes the region of interest but leaves an FRT- attP−flanked RMCE cassette marked by the w + and y + visible markers.

The version of the RFP-GFP cassette marked with hphMX instead of kanMX (in strains JRY9729 and JRY9730) was constructed by transforming strains containing the kanMX-marked RFP-GFP cassette with PCR product amplified from pAG32 (Goldstein and McCusker, 1999) using the P TEF fwd and T TEF rev primers.

you can find the gear ratio as it is marked on the cassette or you can count the teeth on the top gear (largest sprocket) and the teeth on the bottom gear (smallest sprocket) and this will tell you the gear ratio.

Using a GFP-marked cassette, they observed 7.9% GFP-positive animals in the first generation, which went to 0.5% of 6014 animals in the second generation.

As described below, our strategy was to use traditional P-element mediated transgenesis to incorP-element mediatedmarked RMCE transgenesistoes onto balancer chromosomes.

LoxP sequences flanking the neomycin selection cassette are marked by triangles.