The image appeared black due to no nanobubbles); however, when the nanobubbles were injected in rabbit, marked and complete power Doppler enhancement appeared immediately following slow infusion of the contrast agent and color flare appeared in the renal parenchyma).
Fourth, we marked the public health emergencies and injected simulated outbreaks in data sequences.
Challenger fish were previously marked by a fin clip and injected with 5 ml of ~3 × 10 TCID50/ml of IPNV (strain V-1244 cultured at the Norwegian School of Veterinary Science).
To this end, we used the transgenic zebrafish line Tg(sox10 7.2):mrfp), which marks migratory neural crest cells [31] and injected sec24d, sec24c, and combined sec24d plus sec24c morpholinos (Figure 9 and not shown).
Experimental and control individuals were reared from birth in a closed system at a constant salinity of 10 and injected with oxytetracycline (OTC) to mark the otoliths at the beginning of the experiment.
Animals were sexed, measured, weighed, individually marked with subcutaneously injected passive identification transponders (ID100 Trovan, EURO I.D. Usling GmbH, Weilerswist, Germany) and equipped with collars for temperature measurements (see below).
For in vivo lung extravasation assays, CellTracker™ Green (Invitrogen -marked cells (5 × 10) were suspended Invitrogen -marked1× PBS and injecellsinto the tail vein.
After the last recording session, four recording sites were marked by injecting alcian blue to provide orientation points to check the location of the electrode tracks in the forebrain [e.g. 19], [39], [40].
To express α1a receptors, a cDNA plasmid encoding the receptor (provided by Dr. John Pediani, Glasgow University; see Pediani et al. 2000) was injected into individual cell nuclei 4 h after initial plating, together with a cDNA plasmid encoding the jellyfish enhanced green fluorescent protein (eGFP) to mark the injected neurons.
"I was restrained and injected a lot.
