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Asterisks mark the presynaptic neuron.
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In this context, we defined presynapses as spots immunoreactive for the presynaptic marker synapsin and post-synapses as spots immunoreactive for DLG4, essentially marking dendritic spines (Sorra and Harris, 2000).
Immunohistochemical analyses showed loss of the presynaptic marker synaptophysin [ 123].
Immunocytochemistry colocalizes the gene product specifically with the presynaptic marker.
We tested this by triple staining the first instar NMJs with anti-HRP antibodies to visualize the presynaptic nerve, NC82, an antibody that marks presynaptic active zones [ 38], and anti-GluRIID antibodies [ 26], which label all postsynaptic glutamate receptors.
Limiting the number of rendered objects speeds things up, which is why we chose to limit how deep into the presynaptic terminal we rendered".
The neurotransmitter is terminated by uptake into the presynaptic terminal.
These data confirm the presynaptic expression of synaptic homeostasis and implicate presynaptic CaV2.1 in a homeostatic retrograde signaling system.
When the presynaptic spike appears before postsynaptic spike, Δt > 0 (Fig. 4b I); when the postsynaptic spike occurs before presynaptic spike, Δt < 0 (Fig. 4b III).
They change according to the activity of the presynaptic cell and together determine the synaptic strength.
LTP is believed to be strongly related to the activities of the presynaptic and postsynaptic neurons.
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