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TCRVβ expression was determined with the IO Test Beta Mark kit (Immunotech), following the manufacturer's instruction.
The TCR-Vβ usage was determined by the IOTest1 Beta Mark kit (Beckman Coulter, Marseille, France).
To this end, flow-cytometric analysis was performed using the IO Test Beta Mark kit (Beckman Coulter, Brea, CA, USA) as described.
The Vβ-TCR repertoire was examined using the Beta mark kit (Beckman Coulter), 5 × 10 cells/tube being stained in 10 μl of the eight different vials containing mixtures of FITC, PE, and dual-color FITC-PE-conjugated TCR Vβ antibodies.
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Pollen-carrying bees were captured upon return from a foraging trip at the hive entrance and briefly immobilized on ice, so that a RFID tag with known id number could be glued to each bee's thorax with shellac glue from a queen marking kit.
The probes for northern blotting were labeled with [g-32P]ATP by the use of mirVanaTM Probe & Marker Kit (Ambion).
The oncogenic risk of the HPV types detectable with CE marked and IVD marked kit of Genomica are according to the Munoz et al [ 12].
Expression of the miR-16 effector sequence was detected using the mir-16 RNA probe provided with the mirVana Probe & Marker Kit, (Ambion).
Caspase activation was quantified using the CaspACE™ FITC-VAD-FMK In Situ Marker kit (Promega, France) according to the manufacturer's instructions.
For the gfp experiments, first mRNA of gfp was in vitro transcribed using the T7 High Yield Message Marker Kit (Ambion) according to the manufacturer's instructions.
An RNAse protection assay (RPA) was conducted to detect expression of shEGFP (mirVana Probe & Marker Kit, Ambion) using the RNA probe LL91 as described previously [ 14, 17].
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