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Koike, R. et al. The splenic marginal zone is absent in alymphoplastic aly mutant mice.
For these cells, migration through the marginal zone is linked to the development of their characteristic layer 1 axonal arborization.
In addition, the organization of both the white pulp and the marginal zone is under strict control of lipid mediators and adhesion molecules, as well as chemokines, all of which help the specific cellular subsets to be retained within their compartments.
The marginal zone is a tissue compartment surrounding the PALS and lymphoid nodules and is predominantly composed of intermediate-sized lymphocytes and various macrophage populations.
It is likely that the cell cycle in the dorsal marginal zone is regulated through locally-acting mRNAs or proteins that require activin signalling for their expression or appropriate post-translation modification.
Thus, cVg1 expression in the marginal zone is both necessary and sufficient to initiate formation of a primitive streak.
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Moreover, detailed analysis of Plxnd1-eGFP showed that all eGFP-positive cells and processes in the marginal zone were double labelled with Reelin or CALR, which ruled out the expression of Plxnd1 in neurons other than CR cells in this layer at these developmental stages in dorsal pallium (Fig. 1g o).
Examination on sections confirmed the vz discontinuities (therefore similar to PCNA, compare to Fig. 1) in the A/P axis, and additionally revealed a complex pattern in the depth of the neuroepithelium (Fig. 2Q): both the ventricular and the marginal zone were positive for these two clones, leaving a non-expressing neuroepithelial zone at the level of the putative svz.
The marginal zone was located superficial to the cortical plate, adjacent to the pial surface.
The marginal zone was characterized by intense MAP2, GAP43 (Fig. 3) synaptophysin and vGAT immunoreactivity (Fig. 4).
After hemalaun and eosin staining, areas of tumor cells within the splenic marginal zone were selectively microdissected and the tissue was directly transferred into DNA lysis buffer.
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