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For "marginal" targets (i.e. 3 occupancy units or less), we required that at least 2 of the 3 replicates give greater than 2.5 occupancy units to avoid artificial inflation by a single replicate.
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Researchers at InFacts, a group that campaigns against a "destructive" Brexit, studied a dozen new candidates selected to fight in seats where an existing Tory is standing down, together with those in the 50 closest marginals targeted by the party.
After quantile normalization of the raw data, lncRNAs and mRNAs that at least 5 out of 10 samples have flags in Present or Marginal (All Targets Value) were chosen for further data analysis.
After quantile normalization of the raw data, lncRNAs in which all 7 samples displayed flags corresponding to Present or Marginal ("All Targets Value") were selected for further data analysis.
After quantile normalization of the raw data, mRNAs where at least 5 out of 6 samples had flags in present or marginal ("All Targets Value") were chosen for further data analysis.
After quantile normalisation of the raw data, lncRNAs were all detected in each group; three samples having flags in Present or Marginal (All Targets Value) were chosen for further data analysis.
mRNAs and lncRNAs that had flags in the present or marginal ("All Targets Value") in both samples were chosen for fold-change comparisons, and the differentially expressed mRNAs and lncRNAs with a fold change of ≥2 were identified for further analysis.
In the second approach, the PC representation is obtained by having recourse to the Rosenblatt transformation and simultaneously matching a set of target marginal pdfs and target Spearman's rank correlation coefficient (SRCC) matrix.
He repeated the trick two years later when Howard won with marginal seats targeted by Crosby.
Meanwhile, a letter signed by 100 Labour candidates - including many in key marginal and target seats - called for an end to the "campaign of briefing to destabilise Ed and his leadership".
A set of properly ordered conditional pdfs, that uniquely characterizes the target joint pdf, in the first approach and a set of target marginal pdfs and a target SRCC matrix, in the second approach, are estimated from available experimental data.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com