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The estimated marginal means are also reported with 95% confidence interval (CI).

The responses evaluated with this model are shown graphically in Fig. 4A (left side); estimated marginal means are shown in Fig. 4B (left side).

Covariates for the estimated marginal means are Target Duration and Repetition (as per LMM analysis).

Marginal means are presented for each model, representing the mean values for each SF-36 scale after adjustment for covariates.

Estimated marginal means are presented in Additional file 2. Post hoc analysis showed that PT was lower in PWS compared to both O and H (p < 0.001) at all speeds, and in H compared to O (p < 0.001).

Estimated marginal means are presented in Additional file 3. Post hoc analysis showed that PT%BW was lower in PWS compared to both O and H (p < 0.001) at all speeds, and in O compared to H (p < 0.001).

Then, pair wise comparisons between estimated marginal means were done using Bonferroni adjustment for multiple comparisons.

P-values are for trend between all three genotype groups and pairwise comparisons based on estimated marginal means were adjusted for multiple comparisons with Bonferroni correction.

Confidence intervals for the estimated marginal means were computed in the R programming environment, version 3.1.1.

95% confidence intervals for predicted marginal means were calculated using the delta method.

Marginal means were estimated from the model including all statistically significant (P < 0.050) variables.