To eliminate transcripts with only marginal expression, the expression level of the transcripts had to be above 10 TPM in at least one group of samples (control or treated) in order to be included in subsequent bioinformatics analyses.
However, the model does not describe the data quantitatively for lower IPTG concentrations up to 5 μM (Additional file 1): this regime shows only a marginal rise in expression levels, and hence only a marginal increase of cost and benefit terms is predicted by the model, which contrasts with the measured cost and benefit that show significant increases.
In other words, the overlap of genes with marginal statistical support for an expression level difference between individuals with low or high lymphocyte counts on the one hand, and loci with marginal support for a genetic association on the other hand, would be no better than that expected by chance alone.
Lateralised sensory input through antennal amputation has only a marginal effect on expression levels of NrxI and NLG2 5, but a more pronounced effect on expression levels of NLG1.
From the growth data we indeed expected a low selective pressure, as it indicates only a marginal difference in fitness between the expression level at 15 μM IPTG and the optimum that lies nearby at somewhat higher IPTG levels.
However, the relative expression level of TIMP4 is marginal compared to the other 3 TIMPs (Fig. 6C).
No elevated expression level of IL4 and only a marginal up-regulation of STAT6 after allogeneic transplantation were found so that the function of SPI-C remains unclear.
The probe-pair (PM-MM) data were used to detect the expression level of genes on the array (present call, marginal call, and absent call) by MAS 5.0 (Wilcoxon signed rank test).
For each probe set, absolute analysis generates a signal value (expression level), a detection call of absent, present, or marginal, and a p-value associated with the detection call [ 11].
