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The Barbados trench mud volcanoes (BT) and the Guiness pockmarks (Gui) from the Gabonese margin were excluded as sharing only one (Gui) or no (BT) species with the other sites.
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Osteophytes and sclerotic margins were excluded from the measurement.
Patients with an R2 resection (macroscopically positive resection margins) were excluded from the analysis.
Patients with close resection margins (⩽5 mm) on surgical specimens or moderate-to-severe dysplasia on surgical margins were excluded.
Patients who had high-risk factors, such as positive pelvic lymph nodes, parametrial involvement, or positive surgical margins, were excluded.
So we selected only patients with primary resected tumors, without prior chemo- or radiochemotherapy (RCTX) – the few patients with postoperative radiation therapy due to positive resection margins were excluded for survival analysis.
11 patients with resection margin positive were excluded.
To ensure adequate representation of the margins, patients with less than three margins available were excluded from the second phase of the study.
Out of the 100 consecutive patients who underwent potentially curative surgery for adenocarcinoma of oesophagus, 6 patients died within 1 month (overall 30 day mortality of 6%) and 4 patients did not have clear (cancer free) resection margins so were excluded from survival analysis.
Patients with preexisting cervical cancer or with positive margins after treatment were excluded.
Patients who died within 30 days of surgery or who had cancer-involved operative resection margins (R1/R2 resection) were excluded.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com