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The resulting maps were tested using the existing inventory.
The resulting maps were tested using existing data and the factors were improved using an iterative procedure until a good agreement was reached.
Three approaches to the creation of gridded population distribution maps were tested.
Group statistical parametric maps were tested for significance using cluster-level inference (cluster-defining threshold, P<0.001; cluster probability of P<0.05, family wise error-corrected for multiple comparisons).
In total, 33 Rubus SSR loci, distributed throughout the seven linkage groups of previously published Rubus linkage maps were tested and identified as heterozygous in the 'Heritage' parental genotype.
To confirm map order accuracy, the AL8/78-RH maps were tested by comparing with:1) a DArT consensus genetic map constructed using more than 100 bi-parental populations [ 23], 2) a RH map of the D-genome of reference hexaploid wheat'Chinese Spring' [ 14], and two SNP-based genetic maps, one with anchored D-genome BAC contigs [ 5] and another with anchored D-genome sequence scaffolds [ 21].
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Eight different chaotic maps are tested and compared in this paper.
The difference between direct observations and distribution maps was tested and shown to give similar results.
The accuracy of the AL8/78-RH maps was tested for both chromosome assignment and marker order by comparing with previously published genetic and RH maps [ 5, 14, 21, 23].
So, the marker order of AL8/78-RH maps was tested by comparison with: 1) a DArT consensus genetic map [ 23]; 2) a SNP based genetic map with anchored D-genome BAC contigs [ 5]; 3) a SNP genetic map anchoring sequence scaffolds of D-genome (referred as Y2280 × AL8/78 map hereafter [ 21]); and 4) a RH map of D-genome of Chinese Spring (referred as CS RH map hereafter [ 14]).
More than five SSR loci in each LG on the 85P map were tested on the NP map.
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