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Proteome maps of wheat albumins and globulins from mature grains of CS and CS-1Sl (1B).
The analysis should be useful in eventual on-line maps of wheat metabolic enzymes in the manner of AraCyc [ 32] or RiceCyc [ 33].
A complementary approach is to construct physical maps of wheat diploid relatives and use the maps for the construction of the physical maps of the wheat chromosomes.
Furthermore, 6,963 wheat EST sequences have been located on physical maps of wheat by using these ESTs as probes in Southern hybridizations [ 15, 16].
Hybridization of pooled BAC-DNA with wheat 40 k unigene array [ 40] was recently used to anchor genes to several wheat physical maps of wheat chromosome arms [ 41- 43].
For example, the physical map of human genome was constructed with the method of agarose gel electrophoresis [ 34], the physical map of soybean genome was constructed with the method of DNA sequencing electrophoresis [ 35], and the physical maps of wheat and Brassica rapa genomes were constructed using the method of HICF with SNaPshot labeling kit [ 29, 36].
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Open image in new window Fig. 3 Developed water economic value map of wheat (average of years 2008 2009).
Somers et al., [25] genotyped 189 bread wheat accessions at 370 loci and 93 durum wheat accessions at 245 loci to examine linkage disequilibrium across the genome, and found that LD mapping of wheat can be performed with simple sequence repeats to a resolution of <5 cM.
They succeed in anchoring of 3,912 ISBP markers to physical map of wheat chromosome arm 1BL.
Thus, it is a powerful tool for the high-resolution mapping of wheat chromosomes [ 29, 38].
DArT markers were also employed in the construction of a physical map of wheat chromosome 3B [ 42].
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