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Our method follows a probabilistic approach applied to the results of fitting protein domains into maps of proteins and the computation of local cross-correlation coefficient measures.
The 5-year, $150 million program is intended to speed up the determination of three-dimensional, atomic-scale maps of proteins, which in turn should accelerate discovery of new drugs by giving pharmaceutical companies a closeup look at the proteins they are trying to target.
Comparison of 2-DE gel maps of proteins isolated from roots treated with P deficiency suggested that more than 13% of the proteins were different in 99038 and Qi-319, which supported the general viewpoint that the P-efficiency of plant was a trait controlled by multi-gene.
All localization density maps of proteins and domains are represented by an isosurface, corresponding to the threshold of 0.15 unless otherwise stated.
Heat maps of proteins with a significant up- or downregulation of at least 25% were created using GENE-E software (http://www.broadinstitute.org/cancer/software/GENE-E/).
Some of these proteins were identified through matching with 2-DE maps of proteins already identified, using bench top MALDI-TOF mass spectrometry.
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Shown are 2D gel maps of protein sulfenation in brain mitochondria isolated from 5- to 30-month-old rats (40 μg protein/gel).
We emphasize how SRM can generate molecular maps of protein complexes and extract quantitative information on the number, size, distribution, and spatial organization of macromolecules.
Although such webs of intracellular interactions are increasingly well characterized, little is known about large-scale maps of protein interactions between cells.
The gplots package from Bioconductor was used to generate heat maps of protein sets [ 16].
Large-scale maps of protein interaction networks have been constructed recently using high-throughput approaches to identify protein interactions [ 151- 155].
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