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The tomato genetic map was used as standard in this work since it shared the most orthologous markers with the maps of potato, eggplant, pepper and Nicotiana.
The work presented here details the integration of the potato reference genome (DM) with a new sequence-tagged site marker−based linkage map and other physical and genetic maps of potato and the closely related species tomato.
At a hundred times larger scale, when genome-wide genetic maps of potato, sunflower, sugar beet and Prunus were compared to the A. thaliana physical map (macrosynteny), syntenic blocks from 1 to 20 Centimorgans were identified.
Here we present the methods of construction and the general statistics of the first two genome-wide BAC physical maps of potato, which were made from the heterozygous diploid clone RH89-039-16 (RH).
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Composed of 2,141 markers, the consensus map constructed in our study constitutes a new valuable dense reference map of potato.
After the publication of the article 'A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome.
This physical map is made from the diploid clone RH89-039-16 (hereaftor referred to as RH), which is the male parent of the ultradense genetic map of potato [ 9].
We describe for the first time in detail the generation of an integrated de novo genetic/physical map of potato and significant refinements to the previously published assembly.
A subset of the candidate genes associated with the methionine content on chromosome 5 could already be placed on sequence data anchored on an ultra high density map of potato [ 42].
The estimated error rate for these maps was thus far lower than an estimated 2.4% level observed in the 10,000 locus AFLP-based map of potato (van Os et al. 2006), demonstrating the high reproducibility of SNP calling with Illumina chips.
This map was compared to the maps of tomato, potato and eggplant in order to elucidate chromosomal evolution within the genus and to contribute to future gene mapping efforts.
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