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Here we discuss the large-scale maps of nucleosome positions made available through recent advances in parallel high-throughput sequencing and microarray technologies.
We have generated genome-wide maps of nucleosome positions in both resting and activated human CD4+ T cells by direct sequencing of nucleosome ends using the Solexa high-throughput sequencing technique.
It is highly visible on heat maps of nucleosome occupancy where experiments are represented as rows and GGRs as columns (Figure 1).
These profiles resemble maps of nucleosome occupancy, suggesting that intrinsic histone DNA interactions are linked to helical rise.
Recently, a number of high-resolution genome-wide maps of nucleosome locations in S. cerevisiae have been derived experimentally.
More recently, genome-wide maps of nucleosome positions have revealed that nucleosomes simply tend to occupy G/C-enriched and A/T-depleted sites [ 22- 25].
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We found that high-level nucleosome occupancy is similar in vivo and in maps of nucleosomes reconstituted by salt dialysis, showing that occupancy at the level of most GGRs is strongly influenced by the sequence of DNA.
We observed similar occupancy and dynamism patterns both in vivo or in vitro under diverse conditions across twenty six high-coverage maps of nucleosomes in the yeast Saccharomyces cerevisiae [1], [2], [6], [7], [8], [9].
We generated maps of nucleosomes harboring inter-strain variation for H3K4me3, H3K9ac, H3K14ac, H4K12ac or H3K4me1.
Genome-wide maps of nucleosomes in a number of eukaryotes have established that RNA polymerase II promoters are nucleosome- depleted but coding regions may have a regular array of statistically arranged nucleosomes [ 2- 8].
To disentangle sequence-dependent nucleosome positioning from the other factors, we have created two high-throughput maps of nucleosomes assembled in vitro on genomic DNA from the nematode worm Caenorhabditis elegans.
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