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Two high-resolution maps of meiotic recombination initiation sites across the genomes of budding yeast and mice illuminate broad similarities in the control of meiotic recombination in these diverse species but also highlight key differences.
Here, we show that colony size data associated with linked gene pairs can be used to build accurate maps of meiotic recombination throughout the yeast genome.
Here, we show that colony size data associated with linked gene pairs can be used to construct accurate maps of meiotic recombination throughout the yeast genome.
Here we show that SGA colony sizes also can be used to obtain global maps of meiotic recombination because recombination frequency affects double-mutant formation for gene pairs located on the same chromosome and therefore influences the size of the resultant double-mutant colony.
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Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.
These results were subsequently confirmed by a genome-wide mapping of meiotic recombination hotspots [1].
A thorough mapping of meiotic DSBs on S. cerevisiae chromosome III at a resolution of 100 500 pb identified only 5 DSB sites in protein-coding sequences out of 76 DSB regions [12].
High resolution mapping of meiotic recombination events demonstrated the existence of BGC in S. cerevisiae (Mancera et al. 2008).
Another complicating factor is self-incompatibility, which makes the production of homozygous genotypes difficult and forces the independent mapping of meiotic products from each parent in F1 progeny.
Furthermore, we created a map of meiotic recombination points in yeast with a yet unprecedented resolution as well as a catalog of chromosomal aberrations.
Fluorescence in situ hybridization mapping of meiotic chromosomes during the pachytene stage is an important tool in plant cytogenetics, because it provides high-resolution measurements of physical distances.
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