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Comprehensive maps of genome variability and population structure would facilitate genome-wide association studies of complex traits, functional gene investigations and the selection of appropriate donors for breeding purposes.
GDB's Internet-accessible files contain descriptions of human genes, maps of genome regions, genetic "markers" to help guide researchers, and 1.5 million "clones --short human DNA sequenclones --shortnto baclones --shortds--thumanroviDNAwhat are called "physequencess" to the genome.
Thank you for submitting your work entitled "A simple method for generating high‐resolution maps of genome wide protein binding" for peer review at eLife.
The following datasets were generated: Skene PJ, Henikoff S, 2015, A simple method for generating high-resolution maps of genome wide protein binding, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE67454, Publicly available at the NCBI Gene Expression Omnibus (Accession no: GSE67454).
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Microarray-based comparative genomic hybridization allows the construction of high-resolution maps of genome-wide copy number alterations.
By deep sequencing cell-free DNA (cfDNA), isolated from circulating blood plasma, we generated maps of genome-wide in vivo nucleosome occupancy and found that short cfDNA fragments harbor footprints of transcription factors.
To visualize this process in molecular detail, we generated high-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural precursor cells into terminally differentiated astrocytes.
This method compares physical maps of genomes with genetic maps by fitting a 4-5 term polynomial functions.
In this paper, we have generated the initial maps of genome-NL interactions in mouse 3T3 fibroblasts and C2C12 myoblasts by DamID assay coupled to high throughput DNA sequencing.
However high resolution maps of genome-wide methylation now show that cytosine methylation occurs throughout the genome, particularly in bodies of highly expressed genes [ 3], and up to 4.25% of cytosines in the human genome are methylated [ 1].
Even extensive sonication only yields fragments with an average length of 200 bp, suggesting that sonication is of limited use in generating high-resolution maps of genome-wide protein binding (Fan et al., 2008).
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