Representative heat maps of cell surface fluorescence intensity per cell for the two libraries screened at 1 µM concentrations are shown in Figure 3C and 3D.
Coupled with two-dimensional gels, MALDI-TOF MS can be used to create proteomic maps of cell types such as macrophages [4] and of intracellular compartments [11].
By comparing the fluorescent microsphere images before and after cell detachment, we computed spatial maps of cell-exerted displacement.
Similar to SPADE, CITRUS identifies clusters of phenotypically similar cells in an unsupervised manner and generates maps of cell subsets based on hierarchical clustering [ 56].
Hence, behavioral imaging with μPET and [18F] fluorodeoxyglucose (FDG) can generate whole-brain metabolic maps of cell-specific functional circuits during the awake and freely moving state.
By contrast, we obtained a contour map of cells in the unstretched and stretched states, enabling an analysis of the height-dependent deformation.
For T2 mapping of cells in vitro, the following parameters were used: point resolution of 156 × 156 μm, section thickness of 0.6 mm, TE = 20, 40, 60, 80, 100, 120, 140, 160 ms, TR = 4000 ms, number of acquisitions =2.
The SERS spectra and SERS mapping of cells indicate that the major Raman bands are mostly assigned to the vibration characteristics of proteins, and the C-H in-plane bending mode of the substituted benzene in Phenylalanine is remarkably enhanced.
This hypothesis has not been fully validated, mainly because a hierarchical map of cells involved in mammary epitheliogenesis has not yet been established.
