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As more SSRs have been developed during the past decade, several SSR-based maps have been constructed, including AA genome and BB genome maps in wild x wild species populations [ 21, 24, 26, 27], and some genetic maps in cultivated x cultivated populations [ 29- 33].
The density of molecular maps in cultivated peanut has been constrained by low molecular polymorphism rates among genotypes and the limited amount of sequence data from which polymorphisms could be mined, although in spite of these limitations considerable progress has been made to develop and integrate genetic maps.
We have shown that GBS is an effective approach for the generation of marker-dense genetic maps in cultivated barley.
In silico analysis of polymorphisms increased the efficiency of polymorphic marker development, and contributed to the construction of high-density linkage maps in cultivated peanut.
In recent years, many efforts have been made to construct linkage maps in cultivated peanut, but almost all of these maps were constructed using low-throughput molecular markers, and most show a low density, directly influencing the value of their applications.
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Shirasawa, K. et al. In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut.
Zhou, X. et al. Construction of a SNP-based genetic linkage map in cultivated peanut based on large scale marker development using next-generation double-digest restriction-site-associated DNA sequencing (ddRADseq).
The results of this study provide 30 novel SSR markers that would be valuable in agriculture via plant breeding, phylogenetic relationships, cultivar identification and linkage mapping in cultivated bottle gourd as well as related cucurbits species.
The development of a dense genetic linkage map in cultivated tetraploid alfalfa is the first step in understanding the genetic control (QTLs) of traits of agronomic interest.
These BES-SSR markers were combined with other publicly available peanut SSR markers to construct a new and higher density genetic linkage map in cultivated peanut, and to initiate the process of integrating physical and genetic map resources in peanut.
This is based on the findings that the useful QTLs from the wild soybean, such as the QTLs for high protein content (Sebolt et al., 2000), SCN resistance (Wang et al., 2001) and yield (Concibido et al., 2003), were already present in the max germplasm and/or were mapped in cultivated soybean populations as well (Wang et al., 2004).
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