The genetic maps has previously been reported in Liu et al. [ 19].
These were taken from six of the eight opossum autosomes, since cytogenetic maps had previously been constructed for wallaby chromosomes 5 and 6q (corresponding to opossum chromosomes 4 and 7 respectively) using the strategy outlined above [ 30, 32].
DOI: http://dx.doi.org/10.7554/eLife.00348.014 Although the DNA methylation system is conserved across vertebrate evolution, CGI maps had previously indicated that this epigenetic system may have significantly diverged between vertebrate species and even acquired unique properties at TSSs during the evolution of warm-blooded vertebrates (Aïssani and Bernardi, 1991; Sharif et al., 2010).
Cysteine derivitization and glycosylation site mapping have previously been successful in elucidating the membrane topology of membrane bound proteins, such as Insig [ 13], and these techniques could similarly be applied here.
The PCR cocktails were then subjected to the following amplification conditions: initial denaturation at 94°C for 4 min followed by 35 amplification cycles of 94°C for 20 s, 48 62°C for 20 s and 72°C for 30 s. Details on the mutation detection strategies and linkage mapping have previously been outlined [ 24, 61- 63].
The field of computerized mapping had previously been booming, but entry-level tech jobs had dried up.
The physical map had previously been generated with fingerprinted contigs (FPC; Soderlund et al. 2000) and SyMAP (Soderlund et al. 2006) using SNaPshot fingerprints and BAC end sequencing (BES) of a ~10× BAC library from O. barthii accession #105608 (see supplementary materials for further data on the BAC library).
QTL mapping has previously been used to determine genomic regions affecting such quantitative traits in other mapping populations, and has identified a large number of QTL [ 4- 7].
Differences of local recombination frequencies (map length) between populations can also effect marker ordering between maps, and the importance of similar recombination frequencies across individual maps when constructing a consensus map has previously been noted [ 35].
Nuclease mapping has previously been used as part of a strategy to map the Xist RepA lncRNA, suggesting a complex 2D structure distinct from the dual hairpin loops which had previously been suggested for Xist RepA.
