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Only unique mappings were counted.
Only unique mappings were counted for expression analyses.
Broken pairs were included in the counting scheme and only unique mappings were counted for expression analyses.
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The "sm" (single-map) invocation ran featureCounts with default settings ensuring only single-mapping reads were counted.
Mapping pairs (from paired libraries) were counted only once.
First, for each strand and position of the genome, all mappings starting at the given position were counted.
All single-mapping reads that overlapped a gene region were counted, and gene regions were defined as the smallest start and largest end position of transcripts annotated to the gene.
Reads aligning to each UCSC transcript were counted at transcript level resolution and then summarized at the gene level using transcript to gene symbol mappings from the kgXref table downloaded from the UCSC Genome Browser at http://genome.ucsc.edu.ucsc.edu
For each read, the number of mappings on the reference is counted along with the lowest number of mismatches found among the various mappings of that read.
The mappings were performed with and without SNP-tolerant mapping.
The mappings were designed to make the Palestinians feel that we are there all the time.
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