Markers on the genetic map were BLASTed against both the BAC-end sequences of the physical map and the genome sequence assembly to anchor the genetic map to the physical map.
Sequences of contigs in the Lucania linkage maps were blasted against medaka sequences using blastn.
In order to link the genetic map to Ensembl Sscrofa9, all 115 microsatellites sequences, which were used in the linkage map, were BLASTed against the Ensembl porcine BAC sequence (Sscrofa9, April 2009).
Marker sequences for the 2,589 SNPs included on the CATIE Type 2 linkage map were blasted to each assembled genome to determine marker position, and all of the linkage groups that contain a mapped marker are displayed with identical markers connected by grey lines (Fig. 8).
Gene models predicted from reference-guided mapping were BLAST searched against Arabidopsis protein data base.
Therefore, the sequences of genetically mapped EST markers were blasted against 1.34x coverage genomic survey sequences of 5DS [ 25] to define intron-exon boundaries; new primer pairs were then designed to amplify sequences from within a single or two closely adjacent exons.
cDNA sequences of genes previously mapped to the region were BLASTed against GENBANK DNA databases to retrieve genomic sequences.
Sequences were imported to Blast2Go software as fasta format [ 80] which were blasted, mapped and annotated using the standard parameters embedded in software.
All clones were two-end sequenced using an ABI PRISM® 3700 DNA Analyzer (Applied Biosystems, Foster City, CA, USA), and their sequences were blasted and mapped according to their positions described in the University of California, Santa Cruz (UCSC) human genome database.
Plouffe's interactive map, which is being blasted to OFA's 13-million person list, helps localize those stories.
