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Using Raman mapping, we were able to determine the chemical composition in various local areas within each sample, and to distinguish and image substantial changes in the distribution of the polymers caused by various preparation parameters.
With only 2 markers remaining unlinked after mapping, we were confident that all of the chromosomes were covered by the map.
Though the resolution of array CGH is much lower than Optical Mapping, we were able to validate 24 structural variants in tumor HF087 and 16 in tumor HF1551 (Additional file 5).
By using an on-line process of concept mapping, we were able to solicit the views of 82 researchers from different regions and local communities in eight countries within a relatively short timeframe and at relatively low cost.
With the help of next-generation sequencing and optical mapping, we were able to assemble a draft of the standard sheep strain of MAP S397 and compare its sequence to other clinical isolates from sheep or the K-10 strain.
By visual inspection of the mapping, we were able to extend the 2nd exon 472 bp upstream of the start codon, define a splice junction spanning 14,481 bp of intronic sequence, and locate a 1,388 bp 1st exon/5'UTR (Fig. 1, Additional file 5: Figure S3).
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"I was interested in scientific cartography and how that becomes GIS [geographic information system] and all the digital mapping we're doing today".
When it comes to projection mapping, we're used to the artwork being of a transient nature.
He is outside time, off the map: "We were nowhere, anywhere — wherever we happened to be".
"When we saw those maps, we were just speechless," said Zuber.
Until it dawned on us that it wasn't actually a map – we were trying to follow the proposed route of the Newbury bypass.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com