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In the fourth simulation experiment, the effect of allelic frequency on QTL mapping was assessed by setting the frequency ratio of the two alleles as 1∶1 (uniform distribution), 1∶2 (skewed distribution) or 1∶3 (skewed distribution).
To proceed to perform translation studies, genome-wide distribution of ribosome footprints (RFPs) mapping was assessed.
The information content of each locus for mapping was assessed by the number of informative meioses (Additional file 1).
The inheritance of these loci was tested via segregation analyses and their usefulness for linkage mapping was assessed in an interspecific cross.
Polymorphism and usefulness of the BES microsatellites for linkage analysis and genetic mapping was assessed in the USDA-NCCCWA panel of five families [ 9] using 193 markers (Additional file 2).
The accuracy of the transcript 5′ end mapping was assessed by comparing it with circularisation RT-PCR or 5′ RACE data, which specifically defines the 5′ ends of transcripts.
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The potential for underground cavity mapping is assessed using synthetic simulations and observations at a test site where the position of underground voids is known.
The predictive accuracy and robustness of mapping were assessed at these feature levels accordingly.
The accuracy of the biomass maps was assessed using available NBS field data.
The level of agreement of the biomass maps with the NBS map was assessed by comparing total and spatial distribution of the biomass estimates.
For the dataset with SNR of 0.11, the resolution of the final density map was assessed to be at 13.5 Å (Fig. 4D).
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