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Another challenge in transcriptomic analysis comes from the ambiguities in read/tag mapping to transcripts.
Reads mapping to transcripts from more than one gene were discarded.
However, the probes mapping to transcripts do not generally lead to new probesets being annotated.
The efficiency of rRNA removal is a key factor to maximize reads mapping to transcripts, because if left alone, rRNAs make up >80-90 >80-90e total RNA of an un-enriched sample [ 18].
To convert the RNA-Seq readatata into a quantitative measure of gene expression, we calculated the number of RNA-Seq reads mapping to transcripts in the RefSeq database (see Methods).
We did not choose "gene-consistent" CDFs (probes of a probe set mapping to transcripts of the same set of genes) to avoid inconsistencies as recommended by Liu et al. [ 23].
Similar(51)
All intronic SNPs were mapped to transcripts and labeled according to whether they fell inside the first intron and by distance from the intron start site.
The KO IDs were mapped to transcripts annotated in AlgaePath.
Exactly how were these sequences mapped to transcripts to identify the exon combinations?
Overall, the majority of variants map to transcripts in both genesets.
Where variation maps to transcripts from both genesets we define the variant annotation as 'concordant'concordant
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