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An additional evidence is strong over-representation in the upper half binomial distribution of unknown protein-coding genes with independent ncRNAs mapping to them [58] (Table 2, "with as-TU" rows).
Since some genes were so small that there were no copy number probesets mapping to them, an additional 10 kb was added to all gene start and stop positions before extracting their copy number.
Relative abundance of the various constructs was derived from the sequence reads mapping to them.
Transcripts that had reads mapping to them from the WT sample were excluded, and we required that the PSR-specific transcripts were abundantly expressed and had at least 50 reads mapping to them.
Repetitive elements are an interesting source of sRNAs, especially in T. pseudonana, where 22% of sRNAs mapping to them.
A further 843 reference genes, with at least one DpnII site, had no tag mapping to them.
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Additionally, there were 343 transcripts that had only one WT read that mapped to them, but these transcripts also had very few PSR reads that mapped to them as well.
It is even providing maps to them, so the curious can seek them out and try them.
Therefore, pages contain only the variable content that has been mapped to them.
Due to the viability of a cross-validation, we additionally excluded 166 protein kinases, which had less than 15 compounds mapped to them.
We compiled miRNA gene promoters from several sources and searched for TFBSs that map to them.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com