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The type of toxin identified by the top BLAST hit to each transcript is shown in column 2. Proteomic evidence is displayed in columns 3 (unique peptides mapping to sequence) and 4 (% coverage of predicted protein with mapped peptides).
a) Number of sequences in set b) HybMap 60-mers mapping to sequence sets.
c) RNA-Seq sequence reads from growth stage mapping to sequence sets.
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Does the probe map to sequence with a SNP at the interrogated CpG?
Then, paired-end reads of each BAC pool were mapped to sequence data set using MosaikAligner.
Composite seeds allow us to develop efficient seed-and-extend aligners for map-to-sequence alignment of erroneous mapping data.
Short reads from RNA-Seq that map to sequences in a junction library are evidence for a splice junction.
Combining these two pieces of information therefore requires effective algorithms to align maps to sequences.
Moreover, 15 of these 30 SNPs mapped to sequences in close vicinity of prophage boundaries.
However, a significant fraction mapped to sequences annotated only as introns (8%) and to regions without annotated genes (intergenic; 6%).
The remaining < 3% of reads mapped to sequences annotated as encoding rRNA and other small RNAs (scRNA, snRNA, srpRNA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com