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After mapping of reads and removal of duplicate mappings (i.e. reads mapping to repeat regions like rRNAs), 15.96 and 2.65 million reads were mapped uniquely to the genome from the whole and the primary transcriptome library, respectively (Table 1).
Subsequently, the percentages of reads mapping to repeat families were calculated (Table 3).
First, it ensures that mate pairs mapping to repeat regions are not used in the scaffolding process even if the repeat region is present only once in the contig collection.
They typically disregard information in reads mapping to repeat sequences, and significant post-processing and manual examination of their output is often required to rule out false-positive predictions and precisely describe mutational events.
Oligonucleotide probes (between 60 and 100 bp) were designed, excluding any sequences mapping to repeat regions or that were not unique in the human genome permitting 80% of the total 1.7 Mb genomic regions to be tiled.
To investigate the extent to which this effect contributed to FPs in our validation set, data on the number of unique mapping reads and on the number of reads mapping to repeat regions were extracted from the mpileup files for each sample.
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The data indicated that about 16 21% of the small RNAs matched with rRNAs, tRNAs, snRNAs and snoRNAs (Figure 2C and 2D), ∼24% mapped to repeat sequences, including transposons and retrotransposons, and ∼1.2% mapped to protein-coding exons.
Of the SNPs identified, 1,832,801 (53%) mapped to repeat containing regions of the genome [ 27, 28].
Consistent with earlier studies, we found that a large proportion of all aligned reads mapped to repeat elements.
After removal of the reads that mapped to repeat regions, including the rRNAs and tRNAs, the remaining 711,851 reads were uniquely mapped either to ORFs or IGRs.
Cloned rasiRNAs were found to be distributed on various chromosomes and mapped to repeat sequences mostly corresponding to retrotransposons in both sense and antisense orientation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com