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Both of these strategies require reiterative mapping to narrow down the relevant interval, which is a time-consuming process.
We used SNP mapping to narrow the critical region containing teg-2 to a 98-kb region on chromosome II containing 18 genes.
To this end they used a clever combination of QTL and haplotype block mapping to narrow the chromatin region carrying the Soc locus down to a DNA segment encompassing about 900 Kb and 22 protein encoding genes.
However, in more challenging cases where the LOH interval is large or complex, we recommend validating linkage by additional fine mapping to narrow the meiotic interval further or reassemble the locus.
James Amatruda (Southwestern Medical Center, Dallas) and colleagues have identified a highly penetrant, dominantly inherited mutation that causes TGCTs in zebrafish and are using interval haplotype mapping to narrow down the locus and clone the mutated gene.
In most cases, gene discovery for QTL is carried out by congenic mapping to narrow down the QTL region to less than 5 Mb, followed by gene expression and coding sequence analysis of each gene in the interval to narrow down a list of candidate genes.
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Best feature: You can redraw the perimeters on the map to narrow your search.
It is possible to search by station name, ID number, lat/lon, etc., and to use the map to narrow the spatial domain as well.
Staffers would then use mapping programs to narrow down which apartment buildings need further field inspection.
Subsequently, we applied a homozygosity mapping approach to narrow the region containing the microphthalmia mutation.
One path involves generating additional experimental mapping populations to narrow an initial, wide QTL support interval [ 1].
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