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The fifth smRNA feature corresponded to the sum of all normalized (TPQ) unique smRNA reads mapping to loci from many pyrosequencing experiments [78], [79], [94], [96], [97] (Datafile S2).
The percentage of mapped reads mapping to loci of annotated RTEs are shown to the right.
We directly replicated five previously reported European variants (P<0.05; mapping to loci harbouring FASLG/TNFSF18, SCHIP1/IL12A, PFKFB3/PRKCQ, ZMIZ1 and ICOSLG).
We show that lncRNAs mapping to loci shared between AID are significantly enriched in immune cell types compared to lncRNAs from the whole genome (α <0.005).
The overall genome-wide SNP patterns in the two P. falciparum populations analysed here were not significantly different, and most of the strong selective signatures were evident in both populations, several of these signatures mapping to loci encoding known targets of antimalarial drugs and immunity.
I would recommend the authors to simply map the contigs or unigenes to the predicted genes to answer basic questions like: 1) How many predicted genes can be detected in the transcriptome? 2) How many new genes could be predicted with the transcriptome data (i.e. those mapping to loci without gene predictions) ? 3) How often do more than one contig map to the same gene model?
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Among trans-mapping effects, only a minority map to loci containing TFs.
By our selection criteria, the two partners of a chimeric sequence map to loci on two different chromosomes.
Simply put, the selection of chimeric sequences consists of selecting queries (EST or mRNA) that map to loci on two different chromosomes.
Of these, seven map to loci where common variants probably influence HbA1c levels via erythrocyte biology.
It maps to loci that have previously been shown to be involved in local adaptation to climate.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com