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Ensembl gene IDs mapping to predicted genes were excluded and the Benjamini-Hochberg False Discovery Rate10 procedure was used to re-estimate the adjusted P-values for Ensembl gene IDs11 mapping to known genes.
Based on the over-represented gene list and their mapping to known and predicted pathways, the test cases of tryptophan, auxin and serotonin biosynthesis pathways were specifically selected for their known association in plant development and response to biotic and abiotic stresses.
Scattered sequence mappings across the full length of hairpin, a strong signal in a limited region that mapped to the loop, and hairpins mapping to known tRNA or ribosomal RNA regions were rejected.
Reads mapping to known miRNA were predominantly 21nt long.
Small RNAs mapping to known miRNAs of other plant species were designated conserved miRNAs in cucurbits.
The reads mapping to known miRNAs were annotated using the miRBase database [ 17].
Similar(31)
The genes in proximity of these markers were explored in the Ovine Genome Assembly OARv3.1, then mapped to known pathways of the Gene Ontology to determine which ones were most represented.
These tags were mapped to known unique Unigene clusters.
In total, around 80% (81.39%–83.82%) of the reads were mapped to known mRNAs, and about 2.3%2.20%2.38%38%) were mapped to known ncRNAs.
Identified polymorphisms were mapped to known cytotoxic T cell epitopes.
In many cases, the regions of highest localized expression mapped to known miRNA loci.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com