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To solve this problem, we present a pipeline that is based on interspecies FLcDNA mapping to genomes, which predicts exon intron structures and the CDSs.
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Summary of mapping results (mapping to genome and genes) from RNA-Seq.
The output from Trinity was first filtered by mapping to viral and ribosomal genomes and then mapped to genomes to obtain transcripts mapped to existing genomes and truly de novo transcripts unmappable to existing genomes; the mapping was performed using GMAP with the same command line options as above.
The binding regions showed no obvious concentration in any specific genomic area when mapped to genome.
Reads mapped to genome represent tags mapped to a unique genomic location without mismatch.
9-13 million reads from each library were mapped to genome.
Probe sequences of the tilling array set were mapped to genome of Arabidopsis TAIR8.
4 '170(39.5%) transcripts were reliably mapped to genome-predicted proteins (E-value <10-10 <10-10
The unmapped reads were then mapped to genome sequence with BLAT tool.
Information and relationships of the sesame high-density genetic map to genome assembly; Table S2.
This ability to move directly from "map to genome to gene" will hasten the identification of genes responsible for traits.
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