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To study fundamental evolutionary questions at the genomic level, a high resolution genetic linkage map is an essential tool for analyses ranging from quantitative trait loci (QTL) mapping to genome assembly and comparative genomics.
Therefore, many of the methods utilized in searching for novel miRNAs, including mapping to genome, scanning potential stem-loop hairpins by computational methods etc., were not applicable to our study.
Summary of mapping results (mapping to genome and genes) from RNA-Seq.
The different insert size between paired reads (1 bp -10 kb for mapping to genome, and < 1 kb for mapping to genes) was set to align the exon-exon junction reads.
Because several steps in CSZ, such as the identification of potential miRNAs by MIREAP/miRDeep2 and reads mapping to genome, were too time-consuming, the development of a web server will be a heavy burden for our computational resources.
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The binding regions showed no obvious concentration in any specific genomic area when mapped to genome.
Reads mapped to genome represent tags mapped to a unique genomic location without mismatch.
9-13 million reads from each library were mapped to genome.
Probe sequences of the tilling array set were mapped to genome of Arabidopsis TAIR8.
The unmapped reads were then mapped to genome sequence with BLAT tool.
Information and relationships of the sesame high-density genetic map to genome assembly; Table S2.
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