Sentence examples for mapping to exon from inspiring English sources

As shown in Figure S2 and Table S1, non-specific mapping to exon junctions is a big problem for RNA-seq.

Transcripts were reverse-transcribed into cDNA followed by PCR (RT-PCR) using a pair of primers mapping to exon 4 and 5 to amplify a 250 bp fragment common to both WT and Q344ter rod opsin transcripts (Fig. 1B).

Complete absence of reads mapping to exon 8 is indicative of a homozygous deletion.

Exon inclusion is computed as the ratio of all reads supporting the inclusion of the exon (reads mapping to exon junctions that include it and the exon itself) to all reads supporting its exclusion.

A selection of cases (N=8) was stained with a commercial antibody BL (A300-263, Bethyl Laboratories, Montgomery, TX, USA), mapping to exon 4, and a newly generated antibody (Zhang et al, 2012), directed against exon 11.

In our simulation, using a criterion with less than 4 nucleotides overlapping will introduce extraordinary non-specific RNA-seq read mapping to exon junctions (data not shown).

For RNAi-mediated BRCA1 knockdown we used a mix of two dsRNAs, mapping to exons 12 and 24.

In addition, a small proportion of sequencing tags could be mapped to exon sequences, which are likely to be RNA degradation products.

These sequences were later mapped to exon 16 or exon 15 of the ErbB4 gene, respectively [10].

A mix of two different dsRNAs was used: BRCA1-A (ID 5479, Ambion) mapped to exon 12 and BRCA1-B, designed with Cenix Designed siRNAs program (Ambion, www.ambion.com/siRNA), mapped to exon 24.

Three gibbon sequences annotated as A3G are clearly A3F-G chimeras – they were compiled by screening of genomic DNA while the break points map to exon boundaries [63].