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The probe annotations were updated by mapping the probe sequences to the current chicken genome assembly (WASHUC 2, May 2006) using the approach as described by Neerincx et al. [18].
Mapping of the Human Genome (HG19): First, we proceeded by mapping the probe sets on the HG19 [ 30].
The Illumina probe annotations were cross-checked by mapping the probe sequence to the NCBI Build 36 genome with MAQ (Li et al. 2008).
Recently, an improved draft genome and annotation was published (The International Silkworm Genome 2008), but a genic analysis of the present microarray data requires mapping the probe set onto the current set of predicted genes (Zha et al. 2009).
By mapping the probe sets to genes, we have identified 991 unique genes differentially expressed at the early time point and 3234 unique genes that are differentially expressed at the late time point.
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In addition to mapping the probes to the genome, we investigated the effect of mapping the probes directly to the set of Ensembl transcripts represented as cDNAs.
We evaluated the discriminatory power of the microarrays by mapping the probes to the predicted ORFs using BLAT [ 21].
We did this by mapping the probes from both platforms to the set of Entrez ids (11751) present on both.
For example Gautier et al have reannotated the Affymetrix HG-U133A probesets by mapping the probes against Human Refseq mRNA using the BioConductor package matchprobes [ 5, 13].
However, several genes/transcripts on the arrays are out of date owing to updates in genome assemblies, causing problems when mapping the probes to new versions of the genome assembly [ 83].
Mapping the probes within each cluster onto the IPA revealed some canonical pathways that were present in the pig specific response to PRRSV infection like Regulation of eIF4 and p70S6K Signaling and Glucocorticoid Receptor Signaling.
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