Sentence examples for mapping the epitopes from inspiring English sources

This method relies on the combination of rational library design, quantitative yeast surface display and next-generation DNA sequencing and was demonstrated by mapping the epitopes of several antibodies that neutralize alpha toxin from Staphylococcus aureus.

We had little success in mapping the epitopes of the POM antibodies using linear short peptide arrays.

The binding of IL-3 to its receptor has been investigated extensively using various approaches, including constructing interspecies chimera [24], [25], mapping the epitopes of neutralizing antibodies [24], [26], and mutagenesis [26] [32].

We further characterised our mAbs by mapping the epitopes that they recognise within the Bcl-G protein.

Traditional site-directed mutagenesis and variations thereof are routinely used for mapping the epitopes of antibodies on target proteins.

Besides this, in the epitope mapping, the epitopes of mAb 8G3 and 10C6 were concluded to be located from the amino acid 275 to 435 on the EA1 protein, and the epitope of mAb 12F6 was exactly located from the amino acid 465 to 554 (data not shown).

To develop mAb-specific binding assays for each antitoxin, we mapped the epitopes of the six mAbs.

Here, we describe a method to precisely and efficiently map the epitopes of a panel of antibodies in parallel over the course of several weeks.

We have mapped the epitopes for inhibitory mAbs 12.8 and 12.10, and blocking mAbs such as 1E1 and 7.5 by using site-directed mutagenesis to change specific amino acid residues in MSP-119 and abolish antibody binding, and by using PEPSCAN to measure the reaction of the antibodies with every octapeptide within MSP-142.

Next, we mapped the epitopes along the respective ebolavirus protein sequences in Fig. 8.

Further insight into LGI protein function may come from studies that map the epitopes within LGI1 recognized by sera of different LE patients.