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The low yield reflects the stringency of the initial mapping, the difficulty of sequencing the 18 nt oligo-dT/dA tract present in each 3′ end tag, and the frequency with which PATs mapped to multiple genomic positions; these latter tags were segregated for separate analysis (see Additional file 1: Table S1).
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Mapping the Unknown The difficulties associated with drawing a clean line between normal and abnormal are not limited to overeating.
Ms. Jarcho is intent on mapping the mysteries and difficulties of partnerships, and every section of "Grimly Handsome" deals with fractious twosomes and uneasy threesomes; three, here, represents something more unsettling than a crowd.
The difficulty in mapping the NOx emission is attributed to the fact that the combustion model operates within the closed cycle period of engines and therefore does not capture the complexity of the charge stratification within the NVO equipped engine.
Widespread application of landscape disturbance and succession models is hampered by the difficulty of mapping the initial landscape layers needed for model implementation and by the complexity of calibrating forest landscape models for new geographic regions.
Neither of the two maps looks complete and the microsatellite map has a lower number of linkage groups than chromosomes, due to the difficulty in mapping the small microchromosomes.
Interestingly, the previously undetermined breakpoint junctions in these two patients have one end within an LCR, leading to large uncertainty regions (~62-230 Kb) in the aCGH data, and difficulty mapping the junctions at sequence resolution for breakpoint junctions.
Because of their annotation as repeats (TAIR8 built) these sequences were automatically omitted from the design of the enrichment array since they would either decrease capture efficiency or introduce difficulties in mapping the sequences back to the genome.
The present experiment overcomes these difficulties by mapping the cortical mechanisms involved in the processing of 3D SfS and 3D SfT in the same subjects using comparable stimuli with appropriate 2D controls.
Because of the low marker density in the existing population-specific linkage maps and the difficulty of understanding the genomic structure of Arachis, two significant integrated consensus maps were recently constructed based on the segregation genotypes of multiple populations, anchored to 20 consensus LGs corresponding to the A and B genomes A01-A100, B01-B10) [ 9, 26].
Figure 1 shows the corresponding person item map, targeting the difficulty of items 1 to 30 to the person ability.
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