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More recently, fate mapping techniques using genetically engineered Cre loxP system have enabled us to examine the fates of NG2+ cells directly in vivo.
This is mainly due to the reduced accuracy of conventional SLN mapping techniques (using blue dye and/or radiocolloids as lymphatic tracers) in cancer types where lymphatic drainage is more complex, and SLNs are within close proximity to other nodes or the tumour site.
This approach provides several advantages over mapping techniques using truncated proteins fused to indicator genes and proteins (32, 48).
There are other methodologies, such as CLAD and probabilistic mapping techniques using Bayesian networks [ 20], that could also be used.
These were amenable to recombination mapping techniques using large multi-generation pedigrees, and allowed the genome to be scanned for linkage using just a few hundred DNA markers.
In the present study, we examine the types of neural progeny generated by NG2 progenitors in the cerebellum by employing genetic fate mapping techniques using inducible Cre Lox systems in vivo with two different mouse lines, the Plp-Cre-ERT2/Rosa26-EYFP and Olig2-Cre-ERT2/Rosa26-EYFP Olig2-Cre-ERT2/Rosa26-EYFP Olig2-Cre-ERT2/Rosa26-EYFP Olig2-Cre-ERT2/Rosa26-EYFP
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Note that the tone and inverse-tone mapping techniques used in this article are the same for all the schemes.
In contrast to classical QTL mapping techniques used in the analysis of complex traits, AM is a method that detects relationships between phenotypic variation and gene polymorphisms in existing germplasm collections, without development of mapping populations [ 9, 10].
Recently, Zhou and colleagues charted Salience Network connectivity disruptions in bvFTD (Zhou et al. 2010), using the same intrinsic connectivity mapping techniques used to define this network in healthy individuals (Beckmann et al. 2005; Seeley et al. 2007b, 2009).
Evaluation methods of homogeneity of Mg ion implanted GaN layer by mapping technique using current-voltage characteristics are demonstrated.
Distribution of perovskite (CH3CH2NH3 PbI3 in the TiO2 film was investigated by a distribution mapping technique using an energy-dispersive X-ray spectroscope (EDS) combined with a field-emission scanning electron microscope (FE-SEM, Jeol JSM 6700 F).
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