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Only unique mapping tags were used for gene expression analysis.
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Mapping tags are expressed on average at 50.78 cpm, while non-mapping tags only at 19.85 cpm.
The number of mapping tags is detailed in italics.
Only uniquely mapped tags were retained.
Mapped tags were grouped into those mapped to the forward strand and those mapped to the reverse strand.
Only uniquely mapped tags were kept for downstream analyses.
It is noteworthy that 92% of these genetically mapped tags were expected by in silico digestion.
Unmapped or redundantly mapped tags were removed from the data set.
Total uniquely mapped tags were normalized to 10 million reads to generate tracks.
Resulting mapped tags were presented as 'wiggle plots' (bedGraph data format) of tag abundance for visualization in UCSC Genome Browser.
In addition, we found that 1,610 previously mapped tags were absent in the current data, and the missing tags were filtered out by the more stringent criteria used in this study that resulted in a removal of 1,649 FL-cDNAs as compared to the previous data set.
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