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Finally, to directly show that these markers could also prove useful for association mapping, we conducted a trial association mapping study using a subset of the genic SSRs to associate with traits affecting wood properties in Populus.
A mapping study using 65 F2 plants generated by the selfing of one interspecific cross A. cepa × Allium roylei individual integrated the SCAR marker SAOE17500 into chromosome 5 as expected.
This was followed by a fine-scale physical mapping study using 4,022 F2 individuals, narrowing the locus to 0.075 cM (150 kb; six recombinants; Xu et al. 2006).
We then undertook a medium-density mapping study using the Illumina Medium Density Mouse Linkage Analysis system [18].
An initial mapping study using approximately 150 markers distributed throughout the murine genome identified a single significant locus on the proximal end of chromosome 2 (Figure 3A; peak at d2mit7, 38.2 Mb).
We surveyed the eight founder strains of the CC and performed a mapping study using 131 incipient lines of the CC.
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Our mapping study used quantitative, statistically testable cytoarchitectonical criteria (Schleicher et al. 2005), to overcome uncertainties caused by pure visual inspection (Brodmann 1909; von Economo and Koskinas 1925; Sarkisov et al. 1949) and intersubject variability.
In addition to the inherent limitations of the individual datasets, there may be problems in combining them: the QTL mapping study used British Charolais and Holstein animals as founders for the experimental crossbred population, while the HapMap SNP diversity study sampled North American Charolais and Holsteins.
As the Scm3 mapping study used formaldehyde cross-linking with sonication (Camahort et al. 2009), it is possible that any Scm3– Cse4–H4 that is loosely bound to chromatin in the vicinity of centromeres would be cross-linked and mapped (Krassovsky et al. 2012).
For these reasons, mapping studies using SSR markers usually include many more markers than those using RFLP markers.
However, a series of recent fate mapping studies using different epithelial and mesenchymal tags showed no evidence of epithelial precursors to myofibroblasts in the kidney and liver suggesting an alternate precursor cell type or that the role of the epithelium in fibrogenesis may be organ or disease specific [64, 73 77].
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