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The mapping strategy used was the double pseudo-testcross [ 49].
The iterative mapping strategy used in primates has not been implemented to sequence other Y chromosomes due to the cost associated with the technique.
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The classic genetic linkage mapping strategy using bi-parental crosses has been widely used to identify R genes and QTLs.
Mapping of SOLiD sequencing reads was performed using a recursive mapping strategy using RNA-MATE v1.1 [ 30] under default settings.
This mapping strategy using the recombinant COMP fragments was applied on each of the 18 mouse-specific mAbs.
comprises a total of 411 sequence characterised markers (SSR, RFLP, EST and gene-specific markers) mapped in the full progeny of 76 individuals, and a further 298 markers mapped using a selective mapping strategy using just six seedlings that divided the linkage groups into a total of 46 mapping bins covering the Fragaria genome.
In the future, positional candidate gene approaches in relating NBS genes to resistance QTLs will be possible once the tetraploid cotton genome is sequenced and the QTL clusters are narrowed to 5 10 cM regions through a high resolution mapping strategy using large genetic populations.
Origin of the 71 anchor markers used for the comparison between the genomes of Prunus and diploid Fragaria using the reference populations (T×E for Prunus and FV×FN for Fragaria) and mapping strategies used: with the whole population ("mapped") or by bin mapping ("bin mapped").
The three complementary mapping strategies used refine greatly our knowledge of chicken microchromosome 16 organisation.
Moreover, standard mapping strategies using recessive markers, although scalable, cannot achieve comparable resolution and often are equally labor-intensive.
Our optical map assembly strategy used a two-pronged approach involving iterative assembly, requiring a sequence reference [ 26], and de novo assembly for dealing with large-scale discordances (sequence vs. map) and gaps in the UMD3.1 build (Fig. 1).
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