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A summary of mapping statistics obtained for each sample is given in Table 1.> -wrap-foot> *read-1 and read-2: forward and reverse primer derived reads.
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These figures show LRseq assembly statistics, obtained by mapping sequenced reads to human genome reference 36.
Statistics obtained by unpaired Student's t test.
SAMtools was used to obtain mapping statistics for the reference sequences, producing counts of the number of reads that map to the spliced and unspliced reference sequences.
Therefore, reads that were erroneously mapped to the reverse-complement strand of the + or − bisulfite converted reference were removed from the mapping file, and the new file was put through the Analysis Pipeline Tool again to obtain the correct mapping statistics.
Regardless of the bisulfite conversion method, excellent mapping statistics to in silico bisulfite-converted references were obtained and methylated CCWGG sites were identified.
From this 3′end data set, reads counts for each library were obtained (see Supplementary file 1 for mapping statistics).
In terms of the dataset, we initially obtained 1,296,283,832 reads, of which 95.75% (1,241,156,436) mapped uniquely to OGS2 (see Supporting Information, Table S1 for individual library mapping statistics).
Sequencing and mapping statistics.
Mapping statistics for freshwater medaka.
Mapping statistics: sRNAs to genome.
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