Sentence examples for mapping specificity and from inspiring English sources

Since each method has intrinsic limitations that may compromise the overall mapping specificity and efficiency, thus more robust sequencing-based methods are required to reliably monitor antisense transcriptomes.

Although our PA libraries can be sequenced directly from the 3′ end, paired-end sequencing was employed in this study, which is expected to further improve mapping specificity and efficiency as well as recapitulate local sequence complexity (e.g. splicing events in the 3′ UTR region).

To capture splicing changes between wild-type and tdp-1 ok803 tdp-1 ok803oly(A)-selected RNA fromutantsstrain was resequenced 100 bpolyired-end A -selectedeA -selected280 million RNAds per sample) to achieve the depth and mapping specifromty to analyzeachternative strainng.

The use of a homoeolog-specific genomic reference instead of a transcriptome reduced mapping ambiguity in two ways; firstly by eliminating chimeric assemblies of similar homoeologous sequences, and secondly by collapsing multiple transcribed variant sequences into a single genomic locus in the reference, thus eliminating redundancy and increasing mapping specificity.

The expected strand for a dataset can be easily configured, and when mapping stranded datasets, output files summarizing mapping results are created for each strand, allowing analysis and visualization of strand mapping specificity.

In this paper, we showed that Minimal Match on Either Side of exon junction (MMES) and the number of mismatches have very strong impacts on mapping specificity.

The mapping specificity of junction reads largely depends on the negative control database.

The results suggested that read length (RL) also has a big impact on mapping specificity (Table S3).

In general, other than increasing MMES and reducing the allowable mismatches, one can also increase the length of RNA-seq read to improve the mapping specificity.

Grouping junction reads according to mismatches is necessary, because number of mismatches have great impact on mapping specificity, especially for shorter reads.

We find that the number of matches per read increases, suggesting a reduction in mapping specificity.